【摘要】 <正> 1978年以食蟹猴疟原虫(Plasmodium cynomolgi)可溶性抗原和硷性磷酸酶抗人IgG结合物进行了疟疾酶联免疫吸附试验(ELISA),发现此种抗原与健康成人血样产生较高的假阳性反应。因此改用培养的恶性疟原虫作抗原进行试验。 材料与方法 1、抗原的制备:虫种由本所病原室从海南岛恶性疟病人分离获得。培养液由RPMI-1640(SERVA Feinbiochemica厂)、HEPES缓冲液、谷氨酰胺和碳酸氢钠配成。用前以含12％AB型血清的上述培养液配制2％的“O”型红细胞悬液,用蜡烛缸法培养至原虫达8～10％,当以裂殖体为主时收集原更多还原

【Abstract】 By using a soluble antigen prepared from material of Plasmodium falci-parnum cultivation in vitro micro-ELISA was carried out for the sere-diagnosis of malaria. With eluates from blood impregnated filter paper of malaria patients, 277 of 307 blood specimens were positive when the absorption value was over 0.4, with a positive rate of 90.23%, Among them 94.25% ( 165/175) and 84.85%(ll2/132)in falciparum and vivax malaria were observed respectively. The false positive rate in healthy individuals from non-endemic area of malaria of Qinghai Province was only 1.21% ( 2/167 ) .For comparing results of serum and dry blood eluates, 23 blood specimens collected from falciparum malaria cases were tested, the positive rates were 91.31% ( 21/23 ) and 95.65% (22/23) respectively. The positive of serum and dry blood eluates in 27 blood specimens from vivax were 66.67%(18/27) and 100%(27/27) respectively. Results suggested that the difference might be caused by drop of serum antibody activity during transport and preservation.更多还原