【摘要】 以1M蔗糖垫超离心法制备大鼠肝聚核蛋白体(Rp),去Rp的上清中再经35～50％饱和度硫酸铵盐析和DEAE-52-氯化钠梯度柱层析制备核糖核酸酶天然抑制物(RⅠ)。紫外光谱和蔗糖梯度超离心分析及麦胚无细胞体系翻译活力测定表明制备过程中Rp基本保持完整,有较高的促进放射性氨基酸参入活力。RⅠ经部分纯化活力提高100倍以上,把它加入到麦胚体系,能进一步提高Rp及兔网织红细胞聚核蛋白体RNA的模板活力。更多还原

【Abstract】 A method of simultaneous preparation of polysomes and the natural RN ase inhibitor from rat liver was presented. The polysomes were obtained with 1M sucrose cushion ultracentrifugation. Results of ultraviolet spectrum analysis, sucrose gradient ultracentrifugation and protein synthesis activity in wheat-germ cell-free system showed that the polysomes maintained their integrity with rather high protein synthesis activity. The RNase inhibitor was isolatedf rom the postpolysomal supernatant and partially purified, its specific activity being increased to more than 100-fold. Addition of RNase inhibitor to the wheat-germ translation system resulted in a significant increase in the template activities of polysomes and polysomal RNA.更多还原