【摘要】 本文介绍一种提取乳过氧化物酶的较简易方法。先用CM-SephadexC-50从牛奶中吸附出粗酶,再经硫酸铵分段盐析,最后用CM-纤维素柱纯化,所得酶制品的A412nm/A280nm比值可达0.95,比活性可达58.6GU/mg,产量常不低于5mg/L牛乳。此酶用于IgG的碘化标记,结果满意。更多还原

【Abstract】 A simplified method for the isolation and purification of lactoperoxi-dase was described. Lactoperoxidase in fresh milk was adsorbed with CM-Sephadex and then fractionated by ammonium sulfate. The crude enzyme was finally purified by CM-cellulose chromatography. The enzyme thus prepared is quite pure. Its A412nm/A280nm ratio usually exceeds 0.9 and its specific activity can be as high as 58.6 GU/mg. The enzyme yield is no less than 5 mg per liter of milk. Human IgG radio-iodinated with this enzyme preparation proved to be satisfactory.更多还原