【摘要】 本文研究了大麦、小麦、黑麦、蚕豆、洋葱等染色体的 N 带带型鉴定技术。采用了两种 N 带技术:第一,将染色体标本在5%三氯醋酸（90℃,6分钟）-0.1NHCI（60℃,6分钟）处理,Giemsa染色;第二,染色体标本经 IM NaH₂PO₄（92—94℃,3.5—8.5分钟）处理,Giemsa 染色。试验证明 N 带技术简单、快速,带型清晰。带型分析表明,N 带并非专一地显示核仁组织者。将上述植物的 N 带与 C 带比较,在有些植物中虽然有些 N 带区与 C 带区一致,但也有些区域仅显示出 N 带而无 C 带或仅显示 C 带而无 N 带。为此,N 带技术与 C 带技术的结合使用,将对染色体鉴别十分有价值。N 带同 C 带一样也是细胞遗传学中一个有用的标记。更多还原

【Abstract】 The present paper is dealing with the investigation of the chromosome N-banding technique and the N-banding patterns in Hordeum vulgare,Triticum aestivum,Secale cereale,Vicia faba and Allium cepa.Two N-banding techniques were applied.First, the chromosome slides were stained with Giemsa solution.Second,the slides were treated in 1M NaH₂PO₄ solution at 92-94℃ for 3.5-8.5 min.After rinsing in tap water they were stained with Giemsa solution.The experiments have demonstrated that the N-banding technique is simple and rapid and the banding patterns are dis- tinctive.The data of N-banding patterns indicated that the N bands did not display the nucleolus organisers exclusively.The comparison of the N-banding patterns of these plants with their C-banding patterns shows that in some of these plants although some regions of N-bands and C-bands correspond,there are a number of instances where regions show N-bands but no C-bands and vice-versa.Therefore,a combination of the N-banding and C-banding techniques should be valuable in the cytological identification of plant chromosome.Like the C-bands,the N-bands are also useful markers in cytogeneties.更多还原