【摘要】 把花粉处于单核晚期的大卫百合[Lilium.davidii var.Willmottiae(wilson)Raffill]花药,放入改良 MS 培养基中,培养基添加适当配比的激素(玉米素2毫克/升,或2,4-D2毫克/升+激动素2毫克/升,或2,4-D4毫克/升+6BA 2毫克/升),在25—27℃室内自然光下进行培养,30天后在花药上长出愈伤组织或胚状体,诱导率可达8.89％,转入分化培养基后70天左右可形成完整的再生植株。通过花粉和根尖压片观察,已见到双核花粉,多核花粉和花粉多细胞球,并观察到愈伤组织和再生植株根尖的分裂相细胞,大多数具12条染色体,证明所诱导的植株是来源于花粉。更多还原

【Abstract】 Anthers with the filament of lily[Lilium davidii var.Willmottiae(Wilson)Rof- fill]were cultured on modified MS medium.Supplemented with different concentra- tions and compatible ratios of growth hormones(Z 2mg/l,or 2,4-D 2 mg/l+KT 2 mg/l,or 2,4-D 4mg/l+6BA 2 mg/l).At this time the pollen grains in the an- thers were at the late uninucleate stage.Anther cultures were incubated at 25—27℃, and illuminated with daylight of about 800—1200 lux.After 30 days,the calli or em- bryoids were produced from anthers.The frequency of the ealli or embryoids induc- tion was 8.89%.After transfer eventually to the differentiation medium,these calli or embryoids developed into plantlets in 70 days. Among the root tips of regenerated plantlets haploid,diploid and aneuploid cells were found,but the haploid cells were produced in about 86.4% of the root tips.It is quite evident that haploid plantlets are derived from the pollen grains.更多还原