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猪血清转铁蛋白类型的测定
DETERMINATION OF SERUM TRANSFERRIN PHENOTYPES IN SWINE
【摘要】 转铁蛋白(Transferrin)是血浆中能够运载铁的一种β球蛋白。已发现家畜血清转铁蛋白都有遗传变异的类型。国外许多调查研究指出,猪、牛、绵羊在转铁蛋白(Tf)型与生殖性能之间有些联系。为了探索我国猪、牛、羊中是否也存在这种联系,结合猪种选育,对江西赣州白猪的转铁蛋白类型进行初步的测定。采取赣州白猪217头的血清,进行淀粉凝胶电泳,其中180头猪的血清转铁蛋白在染色的凝胶上显示多样的带型。Tf型的测定以Hesselholt(1969)所做的经Fe59放射自显影确定的图谱为依据,按电泳相对迁移率计算区带分布位置,初步将这些血清的转铁蛋白区分为AA、BB、CC、AB、AC、BC六个型。从这六个Tf型可以看出赣州白猪中TfB基因最多,TfA基因次之,TfC基因最少,是与血清蛋白质型遗传的规律相符的。
【Abstract】 Transferrin phenotypes have been detected in all domestic animals examined so far. Various investigations abroad have indicated some relationship between transferrin phenotype and reproductive performance in pigs, cattle and sheep. In order to explore such a relationship in Chinese pigs and to apply it to the selection and breeding of swine,the phenotypes of scrum transferrin were examined in 217 samples from Ganzhou White pigs of Jiangxi Province by the starch gel electrophoresis technique. Protein in 180 samples occurred in various molecular forms exhibiting multiple band patterns on the stained gel after electrophoresis. According to Michael’s work in which transferrin phenotypes were identified in starch gel eletrophoresis by autoradiography with Fe59 The locations of the bands were determined by electrophoresis relative mobility. Six transferrin phenotypes (AA, BB, CC, AB,AC, BC) were separated in the Ganzhou White. The gene frequency of Tf BB was the highest among the breeds (including Yorkshire and Taiwan native swine) examined, while that of the TfAA was higher than that of the TfCC. The results obtained were in agree ment with a genetic theory of serum protein.
- 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,1982年03期
- 【被引频次】16
- 【下载频次】31