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中国春小麦(6x)×苏联球茎大麦(4x)属间杂交的研究初报
A PRELIMINARY STUDY ON INTERGENERIC CROSSES BETWEEN Wheat variety AR.CHINESE SPRING(6x) AND HORDEUM BULBOSUM (4x)
【摘要】 本试验选用抗病性较强的球茎大麦(2n=28)为父本,以普通小麦(2n=42)为母本,进行属间杂交,授粉24小时后,在母本柱头上滴赤霉酸,促使幼胚生长,以中国春单体5B 为母本杂交,获得了少量结实的杂交种子;以中国春为母本的,则结合离体培养杂种幼胚,以获得属间杂种。杂种自花不育,而以中国春单体5B 与之回交,获得三株回交一代杂种(B1F1)。杂种 F1与 B1F1代的植株性状有明显的杂种优势; 二者穗部形态结构基本相似,均为穗形细长、有芒似父本,而小穗之多花性则似母本;F1和 B1F1与其父本球茎大麦一样对赤霉病和白粉病等严重的小麦病害具有抗性。观察其染色体数,F1在24~30之间,而B1F1染色体数在45~49之间(其中大多数终变期细胞有20~21个配对的二价体,3~7个单价体)。B1F1代自交与回交均有部分结实。预计经过继续自交和回交,可能在后代中获得抗病的小麦异附加系。
【Abstract】 Some intergeneric hybrids between wheat variety Chinese spring(2n=6x=42)and H.bulbosum(2n=4x=28)were obtained by dri-pping GA3 to the wheat stigmas one day after pollination and then byculturing the young embryos in vitro 12-16 days later.It was found thatthe hybrids were self-sterile,while in backcross with monosomic 5Bof Chinese spring as the male parent,three B1F1 plants were obtained.The morphological characters of F1 and B1F1 manifested an apparenthybrid vigor.Both the shape and structure of spikes of B1F1 were simi-lar to those of F1,the long and awned spikes were like the male parent,and the polyanthous spikelets like the female parent.The awns of F1and B1F1 were shorter than those of their male parent.The flag leavesof B1F1 were longer than those of F1 and their parents evidently.Fur-thermore,both of F1 and B1F1 showed resistance to Fusarium spp.andErysiphe graminis as their male parent.There were 24-30 chromosomesin a number of pollen mother cells of F1 at metaphase Ⅰ;while therewere 45-49 chromosomes(20-21 pairing disomes and 3-7 monosomes)inmost pollen mother cells of B1F1 at diakinesis.when B1F1 was self orbackcrossed to either parent,a partial fertility was obtained.It isexpected that an alienaddition line of wheat may be obtained by selfingand backcrossing through generations.
- 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,1982年02期
- 【被引频次】19
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