【摘要】 分离纯化了AMV-H-10的核酸和外壳蛋白质,测定了核酸的生物活性和分子量。外壳蛋白质分子量约为24,500。四种核酸的分子量:RNA₁-1.3×10⁶、RNA₂-1.1×10⁶、RNA_3^-0.80×10⁶、RNA₄-0.48×10⁶。这数据与Hull所测定的基本相同,但比实际的分子量偏高,文中对此进行了讨论。用电泳洗脱法分离纯化了H-10的RNA_3,4,5;RNA,是卫星KNA还是病毒基因组在提纯过程中的降解产物,有待进一步证明。更多还原

【Abstract】 The viral coat protein and virion nucleic acid were isolated, purified and their molecular weight were determined by gel electrophoresis and analytical ultracentrifugation. The total viral nucleic acid is biologically active. The M. W. of the viral coat protein is about 24,500 dalton, and that of the four kinds of viral nucleic acid were RNA₁, 1.3×10⁶, RNA₂ 1.1×10⁶ RNA₃ 0.8×10⁶, and RNA₄ 0.48×10⁶ dalton which agreed with Hull’s （1969） data, but much higher than they actually are. This controversy was discussed. The RNA₃ RNA, and RNA₅ were purified individually by electrophoresis elution. It remains to be cleared whether RNA₅ is a satellite RNA or just a degradation product of viral genome during the purification process.更多还原