【摘要】 <正> 造血细胞体内扩散盒培养,可以在一个较长的时间内维持造血细胞的功能和形态特点,并能探测各种体液因子和药物对培养细胞的影响。因此,近年来这一技术在研究正常造血细胞的增殖和分化,观察再生障碍性贫血和白血病细胞的生长特点,以及在探索不同药物对培养细胞的影响等方更多还原

【Abstract】 Growth and differentiation of bone marrow cells of .19 patients with blood disorders and of 3 normal subjects were observed dynamically in diffusion chamber culture over a period of 15 days. The results showed that the pattern growth and differen tiation of marrow cells from normal subjects were steady and fairly identical. The average amount of marrow cells of leukemic patients in the diffusion chambers were fewer than that of the normal marrow and some phenomena of maturation and differentiation were observed. In the normal marrow cell culture, nucleared erythroid cells never exceeded 15% and usually disappeared after 10 days. But the proportion of the erythroid series of anemic patients was higher than that of normal, and maintained for a longer time. It is interesting to note that the eosinophils maintained in a higher level and continueus to differentiate and maturate throughout the normal cell culture. This work also confirmed that the eosinophils was originated from eosinophilic committed stem cell (CFU-Eo) which is independent of CFU-C.更多还原