【摘要】 用紫外光差光谱和圆二色性光谱研究了长叶车前花叶病毒HRV_sh在尿素作用下以及经三硝基苯磺酸（TNBS）修饰后外壳蛋白亚基空间结构的变化。TNBS修饰一个赖氨酸残基后的HRV_sh外壳蛋白和HRV_sh完整颗粒的a-螺旋变化很小。空间结构的变化是局部的。讨论了空间结构变化与生物活力的关系。更多还原

【Abstract】 A lysine residue of HRV_sh could be modified by trintrophenylsulfonic acid （TNBS） at pH 8.5 in the absence of urea.After modification,the TNP-HRV_sh lost its infectivity over ninety percent,brt the virus particle still kept its intact form.The CD spectra in the ultraviolet region suggested that the α-helix of HRV_sh was not affected by the modification.Whereas the dif-ference spectra of TNP-coat protein ofHRV_sh showed that there are a peak at 230 nm and two shoulders at 260 nm and 290 nm respectively.The TNP-HRV_sh shows a difference spectra peak at 260 nm and two shoulders at 230 nm and 287 nm.These results indicated that the modification of a lysine residue of HRV_sh with TNBS caused some local alteration of tertiary structure of HRV_sh which led to the loss of its in-fectivity.更多还原