【摘要】 用重结晶法得到了去B链羧端五肽胰岛素(DPI)单斜晶系C2空间群的大单晶。该晶体有两个优点:(1)生成的大晶体是单晶而不是双晶,所以不需要在使用前切割双晶,从而避免了晶体的损伤。(2)衍射能力强,可以得到重原子衍生物的好的反常散射数据。对DPI 的原料进行了一系列的生化分析,发现DPI 原料中有一定量的去六肽胰岛素,这可能是影响晶体生长的原因。经纯化后的DPI 制剂可用重结晶法获得适合于X 射线衍射用的单晶。更多还原

【Abstract】 Large monoclinic single crystals of the pig despentapeptide (B₂₆～B₃₀)-insulin（DPI） have been obtained by recrystallization.They have two advantages:（1） They are single crystals,not twin.Damage of the crystals by cutting wasthus avioded.（2） They show high diffractivity.The anomalous scattering data can be wellcollected.It was found that some deshexapeptide (B₂₅～B₃₀)-insulin was present inthe preparation of DPI.This might be one of the reasons why much trouble has beenencountered in growing the DPI crystals.DPI crystals obtained by recrytallizationare suitable for X-ray diffraction studies.更多还原