【摘要】 本实验用聚丙烯酰胺凝胶电泳分析了两个蜂种的血淋巴蛋白质成分。意蜂和中蜂都是Apis属,血淋巴蛋白质电泳谱相似。但是,两蜂种以及同一蜂种不同级别、不同发育阶段的电泳谱又各有特点。根据实验,我们把成年蜂血淋巴电泳谱分为11条带,雌蜂电泳谱上的带5是卵黄原蛋白;雄蜂卵黄原蛋白含量很少或测不出。 用Thorun方法,在聚丙烯酰胺凝胶平板电泳上测得意蜂卵黄原蛋白的分子量为185,000。更多还原

【Abstract】 The haemolymph protein patterns of two species of honeybees, Apis mellifera and Apis cerana were investigated with polyacrylamide gel disc electrophoresis and were shown to be quite similar. The haemolymph protein pattern of the worker bees of Apis mellifera shows eleven bands typically; there are certain differences in different castes, developmental stages and between the species. Band V of the female bees is vitellogenin which is either absent in the drones or present only in traces. This band of vitellogenin is most prominent in the egg-laying queens. Band XI is a larval protein, most prominent in the larval stage, gradually disappears in the adult stages of Apis cerana, but is still present in the adult stages of Apis mellifera. The hamolyinph protein pattern of the pupa is characterised by having many indistinct bands, probably representing disintegrating proteins during histolysis. The molecular weight of the vitellogenin of Apis mel-lifera was determined to be 185,000 with Thorun’s method.更多还原