【摘要】 <正> 近年来的研究结果表明,采用放射免疫分析技术测定血清结合胆酸,是评价肝胆功能的一项最敏感的实验室指标。特别是在慢性肝炎复发时,血清结合胆酸的升高,要比GPT的改变早几周。轻度肝硬变患者,常规肝功能正常,而血清结合胆酸常升高。因此,测定血清结合胆酸及胆汁酸谱已受到国内外学者的重视。更多还原

【Abstract】 A method of radioimmunoassay for the determination of the serum conjugated cholic acid was reported. The antibodies against BSA conjugated cholic acid were prepared from rabbits. Cholyglycylhistamine was synthesized and characterized. It was iodinated with Na125I by chloramine-T labelling technique, purified by thin layer chromatography and used as tracers in the RIA.The processes of this method for RIA of cholic acid may be summarized as follows: (1) precipitate the serum with 9 vols of absolute ethanol; (2) evaporate duplicate ethanol samples of 50-100ul to dryness; (3) redissolve the residue in 0.01M PBS and incubate the samples in the p esence of antiserum and 125I-iodohistamine cholic acid at 37℃ for one hour; (4) separate bound radioactivity from the unbound with PEG and count the bound part with a gamma counter.Normal sera were found to contain 2. 18±1.3nmol/ml. Accuracy was determined by addition of cholic acid to pooled sera with a recovery rate of 91.5-116.0%; with a mean value of 98.1%. Precision was determined by replicated normal samples, with variation coefficients of interassay and intra-assay as 3.3% and 7.2% respectively. Sensitivity has been maintained to the least detectable amount of 1.25pmol/tube.更多还原