【摘要】 本实验用液态氮制冷,以直径为6毫米的圆形冷冻头(-190℃),冷冻家兔皮肤和横纹肌3分钟,同时测量温度,实验结果表明,冰球平均直径分别为20毫米19毫米。在冰球内部存在着温度梯度,在冷冻后15分钟、1天、3天、7天、14天、28天、42天和70天,分别各处死2或3只家兔,作组织学切片观察,冷冻后3天,冷冻坏死最严重,28天时再生修复基本完成。和前文报道相比较,在同样的冷冻条件下,家兔肝脏的冷冻损伤要比皮肤和横纹肌严重。冷冻损伤后肝脏的修复最为明显。冷冻后70天肉眼观察,可见肝脏留有小的疤痕,但是皮肤和横肌纹留下的疤痕不明显。本研究为临床冷冻治疗提供了实验依据。更多还原

【Abstract】 In the present experiment, the skin and striated muscle in 23 rabbits were frozen for 3 minutes with a liquid nitrogen cryosurgical probe (- 190℃) of 6 mm in diameter. The local temperature of freezing area was measured simultaneously.Freezing over, an ice-ball with an average diameter of 20 mm and 19 mm was formed in each specimen. A temperature gradient inside each ice-ball was detected by the concomitant temperature measurement. To obtain histologic specimens, we killed each time 2 or 3 rabbits after they had undergone freezing and lived up to 15 minutes, 1, 3, 7, 14, 28, 42 and 70 days respectively. A severe cryonecrosis in the treated area was noted on the 3rd days after freezing. 28 days after freezing, the regeneration of the necrosis area of the skin and striated muscle was nearly accomplished.In comparison with our previous findings[1], we found the cryolesion of the rabbit liver was more severely damaged than the skin and striated muscle in the same freezing condition, but the liver recovered much more vigorously than the skin and muscle. 70 days after freezing, we could see with the naked eye a small scar on the liver while the scar of skin and striated muscle was nearly undetectable.更多还原