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文昌鱼原肌球蛋白在胚胎分化过程中出现的时间和位置

TIME AND SITE OF OCCURRENCE OF TROPOMYOSIN IN AMPHIOXUS EGG

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【作者】 吴尚懃吴厚余施奠族

【Author】 Wu Shangchin (S. C. Wu) Wu Houyu and Shi Dianzu(Institute of Oceanology, Academia Sinica)

【机构】 中国科学院海洋研究所中国科学院海洋研究所

【摘要】 <正> 文昌鱼卵子分化较早,根据预定器官形成物质分布图,童第周等认为在8细胞期,中胚层物质分布在植物性半球;32细胞时期,绝大部分在植物性半球的第一层,它构成胚胎肌肉的大部分,小部分在植物性半球第二层,形成胚胎前端的肌节。 Ries,Gersch和Whittaker等认为海鞘卵受精后不久可以看到器官形成物质的分区,用组织化学方法可以测出在这些物质中已有特殊蛋白质的出现,如氧化酶、乙酰胆碱脂酶等。

【Abstract】 The differentiation of Amphioxus (Branchiostoma belcheri tsingtauense Tchang et Koo) egg occurs pretty early. Tung et al (1960) had successfully mapped the distribution of the formative substances during the 8-cell and 32-cell stages respectively. But so far, this has not yet been proved from muscle protein. This paper, therefore, undertakes to prove the time and site of occurrence of the tropomyosin in Amphioxus during their development, using the techniques of double diffusion in agar gel.1. Tropomyosin was extracted from the muscle of adult Amphioxus and the classical crystal was obtained (Pl. Ⅰ:1). Samples showing only one band as tested by the isoelectrofocusing (with gels 5% acrylamide plus 0.135% bisacrylamide included 1% Ampholine, pH 4-10, and with 1% amido black stain, see Pl. Ⅰ:2b) Proves its purity. By means of sodium dodecyl sulfate gel eleetrophoresis, an essential band was found in addition to two high molecular weight polypeptide (with gels being stained with Coomassie brilliant blue R 250, Pl.Ⅰ:2a). Antibody of tropomyosin was obtained from rabbit.2. The embryo of the different developmental stages (spermary, ovary, unfer-tillized egg, blastula and myomere) were homogenized and extracted as antigen of double diffusion. When antibody and antigen were added after 24 hours (25℃), all these occur in immune-precipitation bands apart from spermary (PL Ⅰ:4 and Ⅰ:5). Owing to the complex structure of the ovary, we can not decidedly say from the result mentioned above that tropomyosin was present in the ovarian oocyte.3. The mature unfertilized egg was cut latitudinally to make the ratio of the animal to vegetal hemisphere 1:2, and double diffusion in agar gel, the vegetal half gives positive reaction whereas the animal half is negative (Pl. Ⅰ:6).4. We are of the opinion that the egg of Amphioxus has already its segregation along the A-Ⅴ axis before fertilization.

  • 【文献出处】 海洋与湖沼 ,Oceanologia Et Limnologia Sinica , 编辑部邮箱 ,1982年03期
  • 【被引频次】5
  • 【下载频次】32
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