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瑞氏色素的质量和染色效果
The composition of Wright’s stain and its properties
【摘要】 <正> 瑞氏色素(噻嗪曙红盐)是一个多组分的复合染色剂。过去因无统一的规格要求,产品中各组分的含量变动很大,质量极不稳定,不能保证染色的效果。为了找出瑞氏色素质量不稳定的原因,我们对瑞氏色素进行了剖析和光谱性质的研究,并做了各组分的染色试验。发现其中使细胞核着色的是天青A-曙红Y和天青B-曙红Y。
【Abstract】 1. a. Throrugh analysis and staining tests on each composition of Wright’s stain, an exact composition that really decides the stain effect has been found, it can be used to direct the preparation and purification of Wright’s stain in order to obtain good quality product.b. The main cause hindering the staining effect of Wright’s stain is that the effective composition is too small, or because there are some harmful constituents that affect the result of staining.c. If staining conditions, such as the quality of solvent, pH value, concentration of staining solution, and the duration of time, are all kept optimal, with the guarantee of the purity of the effective constituents, good results of staining can be obtained.2. The composition of Wright’s stain is very important. Staining can be successful only when the stain has the effective constituents. If the main composition of Wright’s stain is incorrect, no matter what arrangement is given to the staining process, the result could only be a failure.Hence, the factors that affect staining may be summarized as fellows:1. The structure of the reagent.2. The conditions for the staining process.
- 【文献出处】 化学试剂 ,Chemical Reagents , 编辑部邮箱 ,1982年03期
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