【摘要】 本文比较了用近交系与非近交系小鼠测定造血干细胞的结果。测定内源性或外源性脾结节时,用近交系小鼠可获得照射剂量或注入的骨髓有核细胞数与脾结节生成数之间的线性关系。而用非近交系小鼠,则缺乏线性关系。用体内扩散盒琼脂培养法测定柱系定向干细胞(CFU-C)时,用两种小鼠皆可获得种入的细胞数与细胞团生成数之间的线性关系。更多还原

【Abstract】 A comparison of the number of colonies harvested was made between the inbredand hybrid strain mice used in the assays of hemopoietic cells,including the numberof CFU-S(exogenous and endogenous)and CFU-C.In inbred strain LACA mice,arather good linear relationship was demonstrated between the number of nucleated bonemarrow cells transfused to the lethally irradiated mice and that of spleen colonies thengenerated in the exogenous CFU-S determinations;there was also a fine linear relation-ship between the dosage of irradiation and the number of spleen colonies in endogenousCFU-S determinations.These relationships were lost when hybrid strain mice were usedin exogeneous as well as endogenous CFU-S determinations.But in CFU-C determinationsa pretty good linear relationship between the nucleated bone marrow cells inoculatedand the number of colonies then appeared was clearly demonstrated no matter whetherinbred strain LACA mice or hybrid strain mice were used.Therefore,in the assay ofCFU-S inbred strain mice must be used,but in that of CFU-C,either inbred or hybridmice may be used.However,if hybrid mice are used for assays of CFU-C,other factors,such as individual variations in the sensitivity to irradiation and reactions to VCR,etc.ought to be considered.更多还原