【摘要】 本文报道了樟叶蜂幼虫的一种病毒病。病毒只在寄主中肠组织中细胞核内复制,故称为肠型核多角体病毒病而有别于鳞翅目昆虫中核多角体病毒病。病虫肛门流出粘液,粘液干后,幼虫尾部就粘附在基质上。因此,感病晚期或已死的幼虫其躯体则头部往下而悬垂于枝、叶上。感病初期,幼虫行动呆滞、食欲不振,体色由正常的绿色逐渐变为棕褐色。 多角体在扫描电子显微镜下观察,为五边形或六边形的多角体,大小0.6—1.0μm,在超薄切片中,多角体的晶格为点线型,点间距为50A,病毒粒子杆状,大小为227×71nm。更多还原

【Abstract】 A midgut nuclear polyhedrosis virus was isolated from a camphor sawfly Mesoneura rufononta (Rohwer) . The diseased insect showed a sympton of losing appetite and thus becoming slow in motion. After infection the color of the larva’s body turned from dark green to faint yellowish brown. The diseased larvae became flaccid, but the skin didnot become fragile.After being infected, the larva usually died in 4 - 6 days. It was observed that the virus-infected larva generally discharges a kind of dark-fluidl through anus, with which the moribund larva might stick to the plant.By the study in the SEM , the inclusion bodies were demonstrated to be a shape of pentagonal or hexagonal polyhedron and measure 0.6-1. 0 μm in size. The examination of an ultrathin section of the midgut NPV inclusion revealed a pattern of regular dots and rows. The lattices of the polyhedra were shown to have a spacing of 50A between the centers of them. The virus particle is of an usual rod shape and measures 227×71nm in size.更多还原