【摘要】 一、改进PAP可溶性复合物的制备方法。采用小量多次注射HRP等方法,增强免疫反应。效果尚满意。 二、改进双桥PAP法的操作流程。采用高度稀释抗血清和延长反应时间,可使背景色浅而阳性物色深清晰。 三、通过对100例各种类型病毒性肝炎HBsAg的检测,证明双桥PAP法似为检测细胞膜HBsAg的较佳方法。更多还原

【Abstract】 Autnors stuoy the applicability of the doulbe bridge PAP method for the demonstration of HBsAg in paraffin-embedded material and compared with those obtained by PAP method.One hundred autopsy cases with various types of virus hepatitis were reviewed. Of these, 72 were positive for cytoplasmlc HBsAg and 68 cases for membranous HBsAg by double bridge PAP method, which is more sensitive than the PAP method, therefore my be a method of choice for demonstrating the membranous HBsAg in liver tissue.更多还原