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几种贫血性疾患红细胞的扫描电子显微镜观察

Scanning Electron Microscopic Observation on Erythrocytes of Several Kinds of Anaemia

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【摘要】 取血一滴,固定、脱水,临界点干燥后扫描电镜观察。将镜下形态异常的红细胞分为五类:口形细胞、嵴形细胞、棘形细胞、中心浅染细胞以及除上诸型之外的异形细胞。正常的红细胞大部份为双凹园盘形,正常人与几种贫血性疾患患者在上五个异常形态组之间的差异均有显著性。PNH与AA症患者在任何异常细胞形态组之间的差异均无显著性。口形细胞往往与溶血相关联。AA症患者有较多的口形细胞,似可验证AA患者有部份PNH细胞这一结论。

【Abstract】 A drop of blood was fixed, dehydrated and dried with critical point drier, and observed concerning its red cells by SEM.The abnormal cells can divide into five types: stomatocytes,cristate cells, echinocytes, pale-staining cells and heteromorphic cells. The red cells of normal person are most of biconcave discs. The differences between each group of five types of normal persons and patients with anaemia are significant. The diffences between each group of patients with PNH and AA are not obvious. The increacing of percentage of stomatocytes is always associated with haemolysis. Patients with AA have more stomatocytes and it seems to have verified the conclusion that patients with AA have small parts of PNH cells.

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