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免疫RNA体外翻译抗体的研究
Antibody Translation of i-RNA in Vitro
【摘要】 <正> 从1960年以来,特别是近十年来,国内外对免疫RNA(iRNA)转输特异免疫功能的研究进行了大量的工作,不仅从理论上研究了iRNA转输免疫功能的特性,iRNA的细胞来源,iRNA合成的动力学,iRNA分子性质以及作用机制,而且已开始应用iRNA于肿瘤和肝炎的治疗,并看到了可喜的苗头。近来Paque等人进一步证明iRNA中起
【Abstract】 Immune RNA was isolated by hot phenol method from spleen of mice immunized with SRBC. Normal mouse spleen cells were incubated with the i-RNA in the concentration of 500 μg/107 spleen cells/ml at 37℃ for 20 min. After washing, and then culturing for 2-8 days in vitro. The SRBC antibody synthesizing capability of these cells was determined by Jern’s plaque hemolysis technique. The results showed that the i-RNA was able to transfer specific antibody synthesizing information in vitro.
- 【文献出处】 北京大学学报(自然科学版) ,Acta Scicentiarum Naturalum Universitis Pekinesis , 编辑部邮箱 ,1982年02期
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