【摘要】 <正> 胃癌是我国最常见的恶性肿瘤,因而建立人类胃癌细胞株对于胃癌的防治具有重要意义。但是胃癌细胞的培养成功较为困难。我国尚未见到胃癌细胞培养建株的报道。更多还原

【Abstract】 During a period of 13 months from May 1979 to May 1980, our group has cultured in vitro the cancerous tissues of 41 patients with carcinoma of the stomach.Ambng them, cells from 3 patients have survived and grown well, and were able to take a new passage once every 7 days. One of the cell lines, "SGC-7901", which has descended for 73 passages begining from Aug. 1979, was presented in more detaiL.The cancerous tissue material was taken.from the paragastric, metastatic lymph nodes. The intra-nodal cancerous tissue was sectioned under sterile condition into small blocks measuring I cubic mm .in size, and then cultured in a medium of RPMI-1640 with 40％ bovine serum. The tissue blocks were placed contact with the giass Wall of the container, and the latter was kept still and undisurbed. The cells proliferated directly over the wall, forming a single-cell layer covering its surface. They were demonstrated to be malignant cells as judged from their morphology, rate of proliferation and mitotic indices. The modal number of the chromosomes was 67, comprising 42％ of the total. Of these, about 76％ had chromosome numbers of 66, 67 and 68 Aberrations were present.When these cells were inoculated to ICR mice subcutaneously, or into the anterior chambers of rabbits, tumors with adenocaroinomatous structure similar to.those in the original metastatic lymph nodes were observed to develop at the inoculated sites. PAS positive granules were seen in the smears, and adenomatous arrangement and architecture were observed both in direct smears and by electron microscopy. Microvilli and secretory granules were also found under the electron microscope as well as intercellular junction complex and interdigiting structures.In view of the fact that the source of the in vitro culture is paragastric metastatic lymph nodes of a patient with gastric cancer, and members of the cell line are proved to be malignant and epithelial in nature, it is concluded that "SGC-7901" is a cell line from human gastric carcinoma.更多还原