【摘要】 本文以蚕豆、洋葱、黑麦为材料,研究了植物染色体的trypsin-Giemsa分带技术。根尖材料经水解、压片、冰冻法分离盖片与载片、空气干燥,用0.025％胰蛋白酶(pH7.2)34℃下处理10—15分钟,10％ Giemsa染色。此法简便、快速,显示出的带纹清晰。文章详细地分析了trypsin-Giemsa显示的蚕豆染色体带型,还对显带机制作了初步讨论。更多还原

【Abstract】 The present paper is the investigation for the chromosome banding technique of trypsin-Giemsa in plants. Vicia faba, Allium cepa and Secale cereale were used. The fixed roots were hydrolysed in 0.1 N HCl or 45% acetic acid and squashed in a drol of 45% acetic acid. Coverslips were removed by the freezing method and the slides dried in the air. The preparations were then immersed in 0.025% solution of trypsin in phosphate-buffered saline (pH7.2) at 34℃ for 10--15 minutes. Staining was perform- ed on 10% Giemsa at pH 7.2. This method has some unique features. It is fast and technically simple and C-banding of good-quality can be consistently demonstrated. In this paper the chromosome banding pattern with trypsin-Giemsa in Vicia faba is analysed and the mechanism of chromosome banding with trypsin-Giemsa is also dis- cussed.更多还原