【摘要】 <正>羊水细胞培养是进行产前诊断遗传性疾病的重要方法之一,它可以预测子宫内胎儿的发育情况,检出胎儿是否有先天性遗传缺陷,以便及早作出合适的处理。 近几年来,国内外文献陆续报道成功地应用羊水细胞培养技术为临床提供新的产前诊断途径。羊水细胞培养方法繁多,国内一般较多采用简易羊水细胞培养方法,但迄今尚未能广泛应用于临床。本室于1979年3月份开始抽取中期引产孕妇的羊水细胞培养。现初步小结如下。更多还原

【Abstract】 This article is made to summarize the types of cell growth, the laws governing morphological changes and time selection for chromosome in the collection process of culture and chromosome collection from amniotic fluid cells. The specimens are from 70 cases of pregnant women with mid-term termination of pregnancy. Experimental results show that the transparent round amniotic fission cells at the height of its division activity are the optimum time for collection of cell chromosomes. In 50 cases of culture which were successful 43 cases had gathering of cell chromosome at such a moment, a success rate of 94%.The principal causes of culture failure in the remaining 20 cases are analysed as follows:1. Bacterial contamination during the course of culture.2. The unsuitable pH of the culture solution (medium).3. The unsuitable date of collection of the amniotic fluid and the pregnancy duration.From our experience in cell collection, and slide preparation of the chromosomes, we put forth suggestion of obtaining more samples which are in the mid-fission stage and are most suitable for analysis.更多还原