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野蚕性信息素研究——化学结构鉴定及生物活性测定
STUDIES ON SEX PHEROMONE OF WILD SILKWORM BOMBYX MANDARIN A MOORE----IDENTIFICATION OF CHEMICAL STRUCTURE AND DETERMINATION OF BIOLOGICAL ACTIVITY
【摘要】 本研究采用Sephedex LH-20凝胶层析柱分离野蚕性信息素粗提物,获得活性馏份。其EAG值达6.8mv,并具田间引诱活性,平均诱捕率为10雄/天/诱捕器。此活性馏份在气谱上有2个主色谱峰,经GC-MS测定得2号色谱峰的分子量为238,基峰m/e 67,M-18=m/e 220,以及其它碎片离子峰完全和蚕蛾醇(反10,顺-12-+六碳二烯醇)一致。以合成蚕蛾醇进行田间引诱试验,亦具引诱活性,诱捕率为2.3~3.5雄/天/诱捕器。证明蚕蛾醇是野蚕性信息素的主成份。
【Abstract】 In this study,an active fraction was obtained by the separation of the crude extract of wild silkworm sex pheromone with the use of Sephedex LH-20 GPC.(Its EAG’s amplitude was 6.8mv,and had attractiveness under field condition.Average of captured rate was 10 males/day/trap.).The active fraction had two major peaks in the chromatogram.GC-MS results indicated that a molecular ion peak at m/e 238,base ion peak at m/e 67,M-18 ion peak at m/e 220 as well as the ion peaks of the other fragments of peak 2 were all identical to Bombycol(E-10,Z-12-hexadecad-ienol).The field test showed that the synthetic compound also had attractiveness for males(captured rate was 2.3-3.5 males/day/trap.).All results indicated that Bombycol was a major component of wild silkworm sex pheromone.
- 【文献出处】 浙江农业大学学报 ,Journal of Zhejiang Agricultural University , 编辑部邮箱 ,1981年03期
- 【被引频次】1
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