【摘要】 <正> 阿托品及东莨菪碱可增加离体大脑皮层薄片乙酰胆碱(Ach)在高钾或电刺激情况下的诱发释放及合成, 此作用很可能是由于阻断了胆碱能突触前受体的负反馈机制所致。樟柳碱是由唐古特东莨菪的根提出的生物碱,其结构类似东莨菪碱,只是在托品酸部分多一个羟基。其中枢和外周作用及其代谢已有报道。关于其对整体动物皮层Ach释放的影更多还原

【Abstract】 Anisodine, a new alkaloid, isolated from the root of Scopolia tangutica Maxim.differs from scopolamine by the presence of one extra hydroxyl group on the tropic acid moiety. The influence of Anisodine on the release and synthesis of Ach by slices of rat brain cortex was studied.Brain slices were incubated in a medium containing the irreversible cholinesterase inhibitor soman and high concentration （25 mM） of KCl and the release and synthesis of Ach were determined by bioassay on the dorsal leech muscle. During aerobic incubation at 37℃ in a medium containing soman with 4.7 mM KCl, Ach was synthesized actively, and both the release and synthesis increased when the potassium concentration of the medium was raised to 25 mM.In a high KCl medium, the addition of Anisodine in concentrations of 8×10^-7, 8×10^-8. 8×10^-9 and 8×10^-10 M, further enhanced the release, and in concentrations of 8×10^-8 and 8×10^-9 M, increased the synthesis of Aeh. However, when the Anisodine concentration was 8×10^-7 M the release of Ach increased, whereas tissue Ach content decreased. This may be due to inhibition of reuptake at high concentration ofAnisodine. The fact that Anisodine at 8×10^-11 M slightly but significantly decreased the content of Ach in the tissue remains to be explained.更多还原