【摘要】 为探讨酯酶染色技术在鉴别T与非T淋巴细胞上的意义,从正常人(26例)外周血分离淋巴细胞,作酯酶染色,同时与E花结试验作对比,以研究两种技术的相关性。结果如下: 1、99.3±2.1％(均值±标准差)的活性ERFC(E花结形成细胞),94.4±7.2％的总ERFC均呈ANAE(+)。 2、ANAE(+)淋巴细胞中,82.4±5.6％具有形成E花结的能力。 3、总ERFC百分率为60.9±8.5％:ANAE(+)淋巴细胞(12例)百分率为56.5±10.5％,二者的差别不显著(P>0.05)。由上可知,ANAE活性和E花结形式成能为常平行存在于T淋巴细胞,二者均可作为T淋巴细胞的标志,酯酶染色由于其方法简便,如能严格控制实验条件,在细胞免疫测定中将获得广泛应用。更多还原

【Abstract】 A study was made for investigating the significance of esterase staining in differentiation of T and non-T lymphocytes. The lymphocytes isolated from the peripheral blood of 26 normal cases were studied with esterase staining, and corresponding rosette tests were made for ascertaining the relationship of these two methods.The results obtained were as follows: 1. 99.3±2.1%(X±SD) of active ERFC (E rosette forming cell) and 94.4±7.2% of total ERFC were found to be ANAE(+). 2. 82.4±5.6% of ANAE(+) lymphocytes exhibited the rosette forming ability. 3. The percentage of total ERFC was 60.9±8.5%, while that of ANAE(+) lymphocytes was 56.5±10.5% (12 normal cases).There was no significant difference (P>0.05). It is apparent that the ANAE activity is always coincident with E rosette forming ability, and both of them will serve as indicators of T lymphocytes. Thus under well-controlled experimental conditions esterase staining will find wide applications in the assay of cellular immunity due to its simplicity and feasibility.更多还原