【摘要】 长叶车前花叶病毒上海分离株外壳蛋白中有二个赖氨酸残基。完整的病毒颗粒或外壳蛋白在pH8.5条件下,只有一个赖氨酸残基与三硝基苯磺酸反应。如果有4M尿素存在,则有两个赖氨酸残基和一个半胱氨酸巯基被修饰。三硝基苯磺酰化的HRV_sh病毒颗粒的感染力丧失90%以上。HRV_sh的外壳蛋白经三硝基苯磺酸修饰后不能与HRV_sh-RNA重建。这些结果清楚地表明两个赖氨酸残基中一个位于病毒颗粒表面,另一个则包在病毒颗粒中。表面那个赖氨酸残基为HRV_sh的感染所必需。更多还原

【Abstract】 There are two lysine residues in the HRVsh coat protein subunit as re-ported in a previous paper.Either the intact virus particles or the coat pro-tein of HRV_sh were incubated with trinitrophenylsulfonic acid at pH 8.5.It was found that only one lysine resi-due was modified with trinitropheny-lsulfonic group.If the reaction was carried out in the presence of 4M urea,two lysine residues and one cysteine sulfhydryl group were simultanouslyblocked.The experiment of infection shows that the TNP-modified HRVsh lost its infectivity over ninety percent.The TNP-protein of HRV_sh has lost its ability to undergo reconstitution with RNA.These results clearly in-dicated that one lysine residue is lo-cated on the surface of the virion and another is hidden inside.The former seems to be essential for the infection of HRV_sh.更多还原