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酶联免疫吸附试验测定过敏性哮喘患者血清中特异性抗粉尘螨的IgE

INVESTIGATION OF SPECIFIC IgE AGAINST DERMATOPHAGOIDE FARINAE IN SERUM OF ALLERGIC ASTHMATICS BY ELISA

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【作者】 陈润芬许以平沈其昀戴舜英黄定九吴家蓉

【Author】 Chen Runfen et al.The Third People’s Hospital,Shanghai Second Medical College,etc.

【机构】 上海第二医学院附属第三人民医院慢性气管炎研究室上海第二医学院附属第三人民医院慢性气管炎研究室上海市第六人民医院

【摘要】 本文采用酶联免疫吸附试验(ELISA)测定了100例过敏性哮喘患者及31名正常人血清中特异性抗粉尘螨的 IgE,其阳性率分别为71%及0%,与皮试的符合率分别为76%及93.5%。39例过敏性哮喘患者同时作放射性过敏原吸附试验(RAST),两者符合率高达94.9%,提示两法的灵敏度相特异性相仿.

【Abstract】 Enzyme linked immunosorbent assay(ELISA)was used to test the serum specificIgE against Dermatophagoide Farinae in 100 cases of allergic asthma and 31 normalcontrols.The positive rate was 71% and 0% respectively,and the coincidence ratewith skin test was 76% and 93.5% respectively.It was found that the age of thepatient,the age of onset of asthma and the history of atopy were all relevant to thepositivity of specific IgE.Among the allergic asthmatics,39 cases had their specificIgE estimated by both ELISA and radioallergosorbent test(RAST)simultaneously,thecoincidence rate was 94.9%.This denoted the similarity in sensitivity and specificitybetween these two methods.

  • 【文献出处】 上海医学 ,Shanghai Medical Journal , 编辑部邮箱 ,1981年11期
  • 【被引频次】3
  • 【下载频次】23
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