【摘要】 <正> 甲醛溶液用于固定和保存各种组织和器官,主要在于其活跃的醛基能迅速与新鲜组织中蛋白质的游离氨基等多种功能基团进行结合,形成桥键,“醛化”蛋白质。不仅灭活各种分解酶,并能防止微生物的污染和腐败。但是长期浸泡于此液中的组织能保存到怎样一个水平,尚未见有报道。现通过对一组封存于甲醛溶液中19～40年的陈旧离体标本的更多还原

【Abstract】 In this study 7 specimens preserved in 10% formalin for 2~4 decades were taken for light microscopy, electron microscopy and histochemical studies for the purpose of investigating the change of human tissues after decades of preservation in formalin. Formalin as a traditional fixing agent could prevent tissues from autolysis effectively, but oould not inhibit the progressive chemical degradation and morphological changes of tissue even though they occurred slowly. Among the tissue components, nucleic acid, lipid, lipoprotein and glycoprotein were found to be easily decomposed, while collagen, actin and myosin could be preserved for a much longer period. Ghost nuclei were present on account of disintegration of DNA, and accumulation of fatty acid was due to hydrolysis of neutral fat. Loss of basement membrane and reticulin fibers of the cutaneous papillary layer owing to degradation of lipoprotein and glyooprotein resulted in desquamation of epidermis which was encountered not infrequently. Thus after decades of fixation, loss of tissue components led to the change in gross configuration, size and color of the specimens verified.Such factors as the duration of fixation, pH of the fixative and tightness of the. sealing, which would be influencing the chemical decomposition and preservation of the tissue, were briefly discussed. The exact role of formalin in reducing the deterioration of tissue conponents and hence the subsequent morphological changes needa further investigation.更多还原