【摘要】 从红皮病猪分离到一株病毒,在PK15细胞系中盲目传代后能够适应,并产生明显的细胞病变,特征为细胞变圆,折光力强,最后自玻面脱落,病毒也能适应IBRS细胞系。病毒悬液用100,000g超速离心沉淀,沉淀物的负染标本在电镜下观察,看到直径20—30nm的病毒颗粒,呈二十面体对称;在喷涂投影标本中则呈球状。5-碘-2-去氧尿核苷不能抑制其生长,吖啶橙染色看到细胞质内存在红色颗粒。病毒对乙醚不敏感,对pH3.0不敏感,50℃60分钟能将其完全灭活,但1M MgCl₂对它有保护作用。病毒在IBRS传代细胞单层培养中于琼脂复盖层下容易形成空斑,在不用中性红的情况下能清晰可见。根椐病毒的主要特征,可以认为这是一种猪肠道病毒。更多还原

【Abstract】 A virus was isolated from pigs infected with "red skin" disease. It could adapt itself to the PK15 cell line after blind passages and producedm arked cytopathic effect, characterized by the cells becoming round, strongly refractive and finally detaching from the glass surface. It could also by adapted to the IBRS cell line. After the virus suspension was centrifuged at 100,000g, the pellet was negatively stained for electron microscopy. The virus particles could be demonstrated with a diameter of 20—30 nm and icosahedral symmetry. When the sample was stained with the metal-shadowing method, the virus particles were almost round in shape. 5-iodo-2-deoxyuridine could not inhibit its growth and when the infected cover-glass culture was stained with acridine orange, red granules were found in the cytoplasm. The virus was ether resistant, aoid（pH 3.0） resistant, and heat labile. It was completely inactivated at 50C for 60 minutes,though 1M MgC1₂ in the medium could procect it. It readily produced plaques on IBRS cell monolayers under the agar overlay, and could be clearly detected without the addition of neutral red solution. According to the main properties, it was suggested that the isolate was a porcine enterovirus.更多还原