【摘要】 在铜绿丽金龟Anomala corpulenta Mots.和华北大黑鳃金龟Holotrichia oblita F.3龄幼虫体内注射标准剂量的日本金龟(虫甲)芽孢杆菌Bacillus popilliae,观察24小时内血细胞的反应。结果表明,昆虫种类不同,血细胞反应的方式也不相同。在敏感虫种铜绿丽金龟中,只有少数个体能在体腔内形成以浆血胞为主的黑核型囊状体;而在不敏感虫种华北大黑鳃金龟幼虫体腔内,大多数个体能形成以珠血胞为主的块状囊状体。血细胞囊状体的形成率大体上同注射感染率相吻合。本文还讨论了囊状体的形成过程及其作用机制。更多还原

【Abstract】 The third instar larvae of Anomala corpulenta and Holotrichia oblita were injected with a standardized dose of spores of Bacillus popilliae Dutky, and the reactions of hemoeytes were examined during the first 24 hours after the injection. Agglomerates of hemoeytes were formed in all the injected larvae and they attached to the fat body and internal surfaces of the body cavity.In the more susceptible larvae of Anomala corpulenta, hemoeytes formed clumps and the cells underwent lysis to yield the melanian acellular substance. The necrotising masses may become encapsulated by plasmatocytes to form capsules with black cores within 5 min., althouth this encapsulation occurred around a few agglomerates. In the nonsusceptible larvae of Holotrichia oblita, most of clumps consisted of spherule hemoeytes, granular hemoeytes and plasmatocytes and the bacteria. The initial aggregetion stage was very rapid, occurring within 5 min., and it was brought about by the release of an adhesive flocculent material by the spherule hemoeytes in contact with the bacteria. Within 24 hours, we did not found any hemocyte lysis and melanization.The result shows that the responses of the hemoeytes of Anomala corpulenta and Holotrichia oblita to the pathogenic Bacillus can give indication of their natural infective rates, and that encapsulation and clump formation can effectively prevent the-development of the bacterial spores.更多还原