【摘要】 对七星瓢虫(Coccinella septempunctata L.)卵子发生过程进行了组织学、细胞学观察及阶段划分,并与取食人工饲料的瓢虫进行对比。卵母细胞仅出现在幼虫期。蛹期已分化为卵母细胞与营养细胞。成虫期卵子发生可以明显的分为卵母细胞分化、卵母细胞营养及卵母细胞卵黄形成三个时期,并分为9个阶段。第1阶段:卵母细胞位于卵原区,进行第一次减数分裂的前期。第2阶段:卵母细胞位于颈区,开始增大,出现了营养索,DNA呈明显的孚尔根正反应。第3阶段:卵母细胞形成卵泡囊并进入生长区,核增大成胚泡。第4阶段:胚泡移至卵质周缘,卵质中RNA丰富,滤泡细胞立方形。第5阶段:胚泡内核仁增大、分枝并释放核仁小体进入卵质。第6阶段:营养索消失,滤泡细胞扁平并出现空位,卵黄形成开始。第7阶段:卵黄球形成逐渐充满卵质,胚泡膜逐渐消失。第8阶段:胚泡消失,滤泡细胞开始分泌卵壳。第9阶段:卵发育完成,经过上皮塞进入输卵管。取食人工饲料瓢虫的卵子发生过程显著缓慢,发育中的卵母细胞致量少,滤泡细胞及卵黄分布均不正常。更多还原

【Abstract】 The present paper is a report on the oogenesis of Coccinella septempunctata L., the seven spotted ladybird beetle in natural condition and that fed with artificial diets.Oogonia were found early in the larval stage; they grew into oocytes and nurse cells in the pupal stage. Oogenesis in the adult stage could be divided into 9 stages:1. Oocytes were located in the germarium; first meiotic division occured at this stage.2. Oocytes grew in size and entered into the neck region; nutritive cord appeared. DNA showed a positive Feulgen reaction. 3. Egg chambers were formed in the vitellarium. The nucleus of the oocyte transformed into germinal vesicle. 4. The germinal vesicle of the oocyte moved into the periplasm, RNA in the ooplasm is rich, and follicular cells became cubic in shape. 5. Nucleolus increased in size and sent out branches; nucleolar bodies were released into the ooplasm. 6. Nutritive cord disappeared, follicular cells became flattened and open spaces appeared between them. Vitellogenesis began at this stage. 7. Ooplasm was gradually filled up with yolk granules, and the membrane of the germinal vesicle began to disappear. 8. Germinal vesicles has completely disappeared follieular cells began to secrete egg shells. 9. Oogenesis completed. The mature egg cell passed through the epidermal plug to enter into the oviduct.The process of oogenesis in the seven-spotted ladybird beetle fed with artificial diets was comparatively slower, and developing oocytes were fewer in number. The follicular cells and yolk granules were abnormal.更多还原