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肺毛细血管和肺泡铸型的扫描电子显微镜观察

OBSERVATIONS OF THE PULMONARY CAPILLARY AND ALVEOLAR CASTS UNDER THE SCANNING ELECTRON MICROSCOPE

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【作者】 侯广棋魏宝林张朝佑韩云明廖瑞雷琦袁桂琴应国华李向印赵玉珍

【Author】 Ho Guangqi, Wei Baolin, Zhang Chaoyou, Han Yunming, Liao Rui, Lei Qi, Yuan Guiqin, Ying Guohua, Li Xiangyin, Zhao Yuzhen(Hebei Medical College, Shihchiachuang)

【机构】 河北医学院解剖教研室河北医学院解剖教研室 电镜室电镜室

【摘要】 本文用3例雄性成年杂种狗的肺,以ABS丁酮溶液灌注。其方式有三种:1.肺动脉;2.支气管;3.肺动脉和支气管同时灌注。制成铸型标本后,取一小块放入Eiko IB-3型镀膜机内喷镀,用日立S-450型扫描电镜观察,结果如下: 在肺血管铸型扫描中,观察到围绕肺泡的密集毛细血管网的立体构筑图象。毛细血管网为单层,网由五边和六边的毛细血管环组成。网眼的直径往往比毛细血管本身的管径要小。在肺毛细血管和肺泡铸型扫描图象上,显示了胸膜下肺泡毛细血管,特点是毛细血管网比其余部分的网眼宽大,可清楚地看到肺泡周围毛细血管的形态和位置。肺泡呈多面体形,大小不一,表面光滑,其上可见肺泡Ⅱ型细胞核的压迹。两肺泡之间的桥形结构是肺泡孔,其数目和孔径不等。对肺泡孔的机能略加讨论。

【Abstract】 Three fresh lungs removed from three male adult dogs were injected with ABS. This material was injected into each lobe of the lung through the pulmonary artery and bronchus simultaneously. The pressure was maintained between 200~280 mmHg. The injected specimens were digested in 20~50% HCl and peptic solutions for 7~10 days. A part of the cast replicas of the lungs was taken off and gilded with EIKO IB-3. Under SEM (HITACHIS~450) the specimens thus prepared were observed. The chief findings were as follows:Scanning electron micrographs of the injected vascular system presented a clear, three-dimensional picture of extensive capillary networks around the individual alveoli. In the septa between alveoli the capillary networks appeared to be a single layer. These networks looked like pentagonal or hexagonal rings; the meshes in between them were smaller than the vessels themselves.The capillaries observed here in the scanning stereopicture belonged to the category of the alvioli cappilary of the subpleura; the meshes of which were larger than those of other parts.Because the numerous alveoli and capillary networks surrounding the alveoli were filled with the ABS, the position and the shape of the capillary networks can be seen very clearly.The lumen of the alveoli were polyhedron in shape, various in sizes and smooth in contour. Many imprints of the alveolar type Ⅱ cell nuclei existed on the surface. The bridge formation between alveoli were really interalveolar pores; their number and diameter varied. The function of the interalveolar pores was briefly considered in this paper.

  • 【文献出处】 解剖学报 ,Acta Anatomica Sinica , 编辑部邮箱 ,1981年04期
  • 【被引频次】35
  • 【下载频次】94
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