【摘要】 本文研究了电离辐射和化学致癌剂对鼠胚原代培养细胞SCE和染色体畸变发生频率的影响。结果说明SCE可以作为检测化学致癌剂的一个比较敏感的指标。电离辐射和化学致癌剂对SCE及染色体畸变的诱发具有明显的不同。这种不同可能与它们致癌作用的机制和致癌作用的强弱有关。此外,本实验说明鼠胚原代培养细胞,在研究电离辐射和化学致癌剂对SCE的诱发中是一个较好的靶细胞。更多还原

【Abstract】 Studies were carried out on the incidence of SCE and chromosomal aberrations in primary cultured cells of mouse embryo exposed to ⁶⁰Co-γ rays and/or treated with chemical carcinogen——benzopyrene.Result shows in the cultured cells exposed to ⁶⁰Co-γ rays at 50 rads, as compared wtih controls there was no statistically significant change in the frequencies of the appearance of both SCE and chromosomal aberrations, In the cells, however, exposed at 300 rads, the frequencies of SCE and chromosomal aberrations were about 1.3 and 24 times higher than that in the unirradiated controls respectively. In the cultured cells treated with 0.05μg of benzopyrene per millilitre of culture medium, the frequency of SCE were increased by about 1.8 times of that of the control group, while the change of chromosomal aberrations had no statistical significance.From the above results, it was suggested that SCE was proved to be a sensitive indicator for evaluating the effects of chemical carcinogens. There is a remarkable difference existing between the frequencies of SCE and chromosomal aberrations induced by ionizing radiation and chemical carcinogens. The difference seems to have a certain relation to their differences in carcinogenic mechanism and effects on carcinogenesis.It was noted that there are some advantages to utilize primary cultured cells of mouse embryo as target cells.更多还原