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血清补体第一成分(C1q)的测定

THE DETERMINATION OF THE FIRST COMPONENT (Clq) OF THE COMPLEMENT

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【作者】 唐慧一朱世乐陈力田高进王永浩

【Author】 Tang Hui-yi Zho Shi-le, Nephritis Research Laboratory of the Department of Medicine, The First Affiliated Hospital, Beijing Medical College

【机构】 北京医学院附属第一医院内科肾炎研究室北京医学院附属第一医院内科肾炎研究室

【摘要】 <正> 补体系统的活化,目前认为有两个途径——典型途径和旁路途径,前者活化顺序是C1、4、2、3、5→9,后者是由一些C3活化因子(备解素,、B因子、D因子、前C3NeF因子、IgG、IgA、frypsin、Plasmin及细菌多醣类毒素等)作用于C3,超越了C1、4、2,

【Abstract】 This paper reports the preparation of the purified Clq (subunit of the 1st component of the complement) from human serum. It has been identified to be pure Clq by SDS-PAGE electrophoresis, the immuno-electrophoresis and others. The rabbit anti-Clq serum produced by immunization with this comparatively pure prodaet reveals only a single line of precipitation. This serum can therefore be used to assay serum Clq by the single radal immunodiffusion method. The normal values of serum Clq determined on 72 blood donors is 241±47μg (ISD)/ml.

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