【摘要】 单一显色剂法测定血清胆固醇,操作简便,出结果迅速,但此直接显色法因血清未经抽提处理受色素类(如胆红素)干扰较大,本文对126例不同程度的黄疸血清用直接单一显色剂法与抽提后磷硫铁法进行平行测定确显示了差异性,当血清胆红素达3mg％时,单一显色剂法所测得的胆固醇含量即已有增高趋势,当达10mg％以上时可使胆固醇含量错误地增高近一倍之多,故建议当血清胆红素>3 mg％时用抽提法测总胆固醇含量。更多还原

【Abstract】 The single reagent method used to estimate total cholesterol in serum is very simple, but some pigments in serum, especially bilirubin, interfere with this estimation. In this article we estimated total cholesteral in 126 icteric sera by both the single reagent and the extracted sulfuric Acid-Ferric chloride methods. When the bilirubin rises to 3 mg% the total cholesterol estimated by the former tends to be higher than that by the later. When the bilirubin is above 10 mg% the total cholesterol estimated by single reagent method in more than 50% higher than that estimated by the extracted method We suggest that when serum bilirubin is more than 3 mg%. total cholesterol estimation should be done with the extracted method更多还原