【摘要】 本文报道了由组织培养产生的山新杨小植株上取得的叶外植体的器官分化及影响它的因素。发现叶外植体带叶柄的下部切段比中、上部叶切段更易产生芽。它们在附加一种细胞分裂素(Z1×10^-6M,2×10^-6M;BA2×10^-6M)和一种生长素(IAA4×10^-8—1×10^-6M;NAA,NAA_oxy4×10^-8M—4×10^-7M)的MS培养基上培养时,芽的分化频率可达70—100％。对诱导芽分化,K是无效的。2,4-D有强烈的抑制作用。ABA,GA₃和乙烯利等其它生长调节物质亦表现抑制作用。由于叶外植体分化频率高,每个外植体可以产生大量的芽,它们又可以通过侧芽分化得到更多的芽,因此这一系统也可能用于山新杨的快速繁殖。更多还原

【Abstract】 This report describe the organogenesis of Populus davidiana ×P. bolleana Loucne hybrid leaf explant from plantlet in vitro and the effects of different factors on its organogenesis. It was found that shoot induction of lower leaf segments with petiole was easier than those of the middle and top segments. When leaf explants were in- cubated on a MS midium with cytokinin (zeatin 1×10^-6 W--2×10^-6 M or benzyladenine 2×10^-6 M) and auxin (IAA 4×10^-8 M--1×10^-6 M or NAA, NAAoxy 4×10^-8-- 4×10^-7 M), the frequency of shoot differentiation would be 70--100%. Kinetin had no activity on shoot formation and 2, 4-D showed serious inhibition. ABA, GA₃, and Ethrel all prevented shoot production. Since higher frequeney of shoot differentiation could be obtained, a lot of shoots were produced on each leaf explant and shoots could be proliferated by axillary buds, so this system could be used for rapid propagation.更多还原