【摘要】 1.牛血清蛋白对被胰蛋白酶消化过的叶绿体光还原 DCIP 的活性有恢复作用。洗涤对经牛血清蛋白修补的胰蛋白酶消化叶绿体的 DCIP 光还原没有影响。说明胰蛋白酶消化后,DCIP 还原位置的蛋白质受损,牛血清蛋白有修补作用。2.CCCP 对胰蛋白酶诱导产生的抑制特性变化可以被牛血清蛋白所逆转。3.牛血清蛋白对胰蛋白酶诱导的叶绿体基粒片层松散现象有部分恢复作用。4.牛血清蛋白对胰蛋白酶诱导的叶绿体光谱在500—640毫微米处吸收下降没有恢复作用。本文提出两个假设:(1)叶绿体膜上的蛋白质存在有微区域性的差异;(2)在类囊体膜上存在有由蛋白质构成的“门”和“通道”的组织。更多还原

【Abstract】 1.Bovine serum albumin stimulates the DCIP photoreduction activity of lettuce chloroplasts which has been treated with trypsin.When these chloroplast preparations were washed by tricine buffer such“reversible action”can still be obtained.It is possible that bovine serum albumin may be incorporated into trypsin destroyed site of the membrane. 2.Trypsin-induced CCCP inhibitory effect on DCIP photoreduction activity is reversed by bovine serum albumin. 3.Bovine serum albumin partially roverses the trypsin-induced unstacking of lettuce chloroplast membranes. 4.After trypsin digestion,there are absorbance decreases around 500—640 nm. Bovine serum albumin has no effect on these absorbance decreases. It is concluded that the membrane-bound protiens responsible for different func- tions of chloroplast are heterogeneous.The results also show that there are gate and channel near the position of PS-Ⅱ on chloroplast membrane.更多还原