【摘要】 利用水平板淀粉凝胶电泳法和盘状聚丙烯酰胺凝胶电泳法,结合酶的染色法研究了小麦种子萌发后的胚、幼根和幼苗中过氧化物酶等6种同工酶及胚乳中淀粉酶同工酶的变化;测定了幼根和幼苗中过氧化物酶和吲乙酸氧化酶的活性。过氧化物酶和淀粉酶的同工酶数目随萌发大量增加。6-磷酸葡萄糖脱氢酶、谷氨酸脱氢酶、异柠檬酸脱氢酶和酯酶在萌发初期的胚中就有不少同工酶带和较强的活性。过氧化物酶、吲乙酸氧化酶和谷氨酸脱氢酶在根中比在苗中活性大得多,相反,过氧化氢酶和6-磷酸葡萄糖脱氢酶在苗中比在根中活性大得多,酯酶在苗中比在根中活性稍大,只有异柠檬酸脱氢酶在根和苗中无甚差异。同工酶带的数目及其相对强度的不同可能与器官差异有关。此研究有助于进一步探讨不同组织和器官在分化时具有不同的代谢特点。更多还原

【Abstract】 Isoenzyme patterns of peroxidase,catalase,glucose-6-phosephate,glutamate and isocitrate dehydrogenases,esterase,amylase and IAA oxidase in the embryos,endos- perms,roots and shoots of wheat seedlings(Triticum aestivum L.var.Nung-da 139) were determined by horizontal starch gel electrophoresis and polyacrylamide gel disc electrophoresis respectively.The number of isoenzymes of peroxidase and amylase was increased with the concomitant increase of days during germination.The isoen- zyme bands of esterase,glutamate,glucose-6-phosphate and isocitrate dehydrogenases in the embryos were more in the begining of germination.The activities of pero- xidase,IAA oxidase and glutamate dehydrogenase in roots were higher than those in shoots.On the contrary,the activities of catalase and glucose-6-phosphate dehydrogenase in shoots were higher than those in roots.However the activity of esterase was slight higher in shoots.There was no difference in the activity of isocitrate dehydrogenase between roots and shoots.The morphological difference of shoot and root is evidently related to isoenzyme patterns.This investigation indi- cates that different metabolic characters are existed in shoot and root during dif- ferentiation.更多还原