【摘要】 从三分三种子萌发的根、茎、叶和花药、种皮诱导的愈伤组织,均含有莨菪碱、东莨菪碱。其含量和生长速度均以茎、叶愈伤组织为最高。根、茎、叶愈伤组织,在培养基中分别加入各种植物激素,培养时表明:BA（6-苄基嘌呤）促进芽的分化,NAA促进根的分化,2,4-D则抑制根的分化,GA₃（赤霉酸）影响很小。获得了三种愈伤组织只分化根不分化芽（加NAA 1毫克/升）和只分化芽不分化根（每升中加BA0.2毫克和2,4-D 0.2毫克）的良好结果。分化的这些芽或根的诱导频率,以茎、叶愈伤组织为高。分化的芽或根中均含有莨菪碱及东莨菪碱,但其含量相应地比它们的未分化的愈伤组织约低1～4倍。薄层层析结果表明,愈伤组织中有6种生物碱,茎、叶愈伤组织中,还有一种在紫外光下显蓝色荧光的物质,而分化的根或芽及花药愈伤组织中只有4种生物碱。看来,在离体培养下的三分三愈伤组织是有莨菪碱和东莨菪碱合成的全能性。这两种生物碱似乎并非最终的代谢产物,它们可能参与了器官建成中的代谢过程。文中还讨论了莨菪碱和东莨菪碱的合成部位问题。更多还原

【Abstract】 Hyoscyamine and scopolamine are contained in the callus induced from the root, stem and leaf of seedling of Anisodus acutangulus as well as in the callus induced from the anther and seed coat. Stem-callus and leaf, callus possesed the highest content of hyoscyamine and scopolamine and the highest growth rate.When the root, stem and leaf callus were cultured in LS medium with various hormones added separately, it showed that BA stimulated differentiation of bud, NAA stimulated differentiation of root, 2,4-D inhibited differentiation of root, and GA₃ had a small influence upon callus differentiation. In the experiments, the satisfactory resultes were obtained that the three kinds of callus differentiated roots only （with NAA 1 mg/l added to medium） and vice versa, i.e, the three kinds of calluses differentiated buds only （with BA 0.2 mg/l and 2,4-D 0.2 mg/l added to medium）. Stem and leaf callus possessed the highest inducing percentage of differentiated bud or root, while the root callus had the lowest percentage; all these differentiated buds roots contained hyoscyamine and scopolamineBut the contents of these two alkaloids were 1～4 times correspondingly less than their mother callus.The results of TLC indicated that there were six alkaloids in the callus. Furthermore, in the stem or leaf callus there was a compound which was able to produce blue flourescence under Ultra-Vilet light, while in the differentiated root or bud from root, stem or leaf callus there were only four alkaliods.It appears that the dedifferentiated callus cells possess the totipotency to synthesis alkaloids, and the alkaloids, too, are not the final metabolites. They potentially concern themself in the metabolic prossess of organ-formation. The problem of synthesis sit of hyoscyamine and scopolamine were discussed in the paper.更多还原