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应用反向间接血凝法检测水稻白叶枯病病原

USING REVERSE INDIRECT HEMAGGLUTINATION TEST IN THE DETECTION OF BACTERIAL LEAF BLIGHT PATHOGEN(XANTHOMONAS ORYZAE)OF RICE

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【作者】 王法明陈毓苓邓秀蓉张光洛殷尚智奚文英

【Author】 Wang Fa-ming Chen Yu-lin Deng Xiu-young Zhang Guang-lou Yin Shang-zhi Xi Wen-ying (Plant Protection Institute, Jiangsu Academy of Agricultural Science)

【机构】 江苏省农科院植保所江苏省农科院植保所

【摘要】 <正> 防治水稻白叶枯病,目前虽有一套行之有效的综合防治措施,但由于对病原细菌侵染规律摸得不透,因而在措施上环环都要抓,手续繁琐,急需进一步提高。目前检测水稻白叶枯病菌常用的方法主要有分离培养,噬菌体法、镜检观察喷菌法、浓缩接种法等数种,均有一定的缺点。如分离培养法容易污染,而且标样一经干燥即不易检出;噬菌体

【Abstract】 A reverse indirect hemagglutination test (RIHT) used in the detection of bacterial leaf blight pathogen in leaves and stubbles of infected rice was sensitive, specific and rapid. The Xanthomonas oryzae may be differentiated from X. panici, X. oryxicola, pseudomonas oryzicola and other bacterial contamination within two hours. The RIHT was superior to the bacterial stream observation under microscope and the reaction of bacteriophage examination and was completely identical to the result of the inoculation of rice.

  • 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,1980年02期
  • 【被引频次】6
  • 【下载频次】27
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