【摘要】 花药培养实验共用十九个甜椒品种,在十六个品种中曾获得胚状体,但由于多数胚状体不能正常发育,仅有“四方头”和“旅大”两个品种的花药在附加0.25毫克/升萘乙酸(NAA),1毫克/升激动素,50毫克/升单核苷酸的NT_H培养基(蔗糖3％)上产生了比较正常的花粉胚状体。将其转移到含有0.1毫克/升吲哚乙酸(或0.001毫克/升NAA),2─4毫克/升激动素,4毫克/升6—苄基嘌呤(或10％椰子乳)的NT_H培养基上,花粉胚状体进一步发育为单培体植株(n=12)。花粉胚状体的诱导率因品种而异。低浓度的萘乙酸有利于胚状体的形成,但是不同品种在形成花粉胚时所需要的萘乙酸浓度不同,将获得的花粉植株用0.2％的秋水仙碱液进行处理24小时,其中染色体获得加倍的植株,能开花结果。更多还原

【Abstract】 Of 19 cultivars of sweet peppers used for anther culture 16 of them gave rise to embryoids, but normal pollen embryoids were obtained only from two cultivars, i.e. "Square Head" and "Lu-tai" ,whereas the embryoids from o-ther cultivars could not develop normally.The basic medium(NTH) was supple-mented with 0.25mg/l NAA,lmg/l kinetin, 50 mg/1 nucleotide and 3% sucro-se. The pollen embryoids were further developed into haploid plantlets(n= 12) after transfer to NTH medium supplemented with 0.lmg/1 IAA (or 0.00lmg/1 NAA) , 2-4mg/l kinetin, 4mg/l 6-BAP (or 10% fresh coconut milk).The frequency of embryoid induction varies with the cultivar. LOW concentrations of NAA are beneficial to embryoid formation but the optimum concentra-tion varies with the cultivar.The haploid plantlets were then treated with 0.2% cochicine solution or 24 hours. Among them, only the diploidized plants gave flowers and set seeds.更多还原