【摘要】 <正> 靛玉红(indirubin)系中药青黛治疗慢性粒细胞白血病的有效成分,目前已用半合成和全合成两种方法进行试制生产,并正在进一步扩大临床实验。为了控制产品质量,我们对靛玉红含量测定方法进行了研究。 文献报道的靛玉红测定方法,我们发现测定结果往往偏高。据推测在靛玉红的合成过程中,可能生成一些靛玉红的同分异构体——靛蓝和靛棕,经硅胶G薄层层析,确证这两种更多还原

【Abstract】 The method of determination of indirubin is described in this paper.Indirubin samples were dissolved in tetrahydrofuran and optical density were measured at 540 nm and 610 nm with model 72 spectrophotometer within 8 hrs. The quantities of indirubin and indigotin were calculated from formula (5) and (6).This determination is not interfered by the presence of indigo brown because of its insolubility.With this method the recovery of indirubin is 98.20±.0.87% and that of indigotin is 98.15±1.87%. The maximum deviation of the determination of indirubin tablets is 0.38～1.21%.更多还原