【摘要】 用偏酸的RPMI1640液培养9例急性粒或粒单型白血病人白细胞,建立了3个非淋巴细胞性白血病细胞系。它们的细胞生物学性质基本相似。 成系细胞的成丛现象、胞间连丝和细胞繁殖都要求一定的细胞浓度,说明它们是以细胞群体存在的。 这些细胞非常活跃,有多种运动方式:用触须样伪足泳动;胞体呈葫芦状收缩或整个细胞拉长收缩前进。更多还原

【Abstract】 We have established 3 non-lymphoblastoid leukemic cell lines from 9 cultures of white blood cells of patients with acute myeloid leukemia (AML) and acute myelomo-nocytic leukemia (AMML). These white blood cells were cultured in acid RPMI 1640 medium. Their cell biological properties are similar.We regard these cells as a population group, which is featured by their clusters, connections between them and proliferation when certain concentration is reached.These cells are very active. Their activities include the following: (1) swim by tentacular pseudopodia,, (2) move about by shrinking their mid-part, and (3) rarely shrinking and elongating.There are a lot of binuclear and polynuclear cells in these cells. Among them micronucleus and micronucleate cells and some pieces of cytoplasma can be seen. Besides typical mitosis, there may exist non-typical mitosis and amitosis.Like leukemia cells in vivo, these cells invasively grow into short-term organ cultures and tissues of immune-deficiency mice.更多还原