【摘要】 用DEAE-Cellulose(DE-11)改进了酶的分离提纯方法,将酶提纯163倍,初步获得电泳均一的酶制剂。酶经光氧化后,活力迅速丧失,其失活过程按一级反应进行。酶经N-溴代琥珀酰亚胺作用后活力逐渐丧失。溴代乙酸在较高浓度下亦能抑制酶活力。酶经酰化后活力亦受抑制。实验结果初步表明:咪唑基吲哚基及氨基都是酶活力所必需的基因。更多还原

【Abstract】 1. The purity of the alkaline phospbata’e of Amphioxus was raised to 163-fold by the u:e of DEAE-Cellulose (DE-11). An enzyme preparation displaying an electrophoretic homogeneity was obtained.2. Photooxtdation of the alkaline phosphatase led to a rapid loss in activity, the decrease in activity was in accordance with the first order reaction.3. The reaction of the enzyme with N-bromsucciniamide also led to a gradual loss of activity.4. Acety ation of the erzyme or reaction with bromoacetic acid in high concentration resulted in the inhibition, of enzyme activity.The above results seem to show that imidazolyl, indodyl and amino groups are essential for the activity of the alkaline phosphatase.更多还原