【摘要】 本文目的在于观察血清孕酮水平与早孕维持的关系。于大鼠妊娠第七天切除卵巢,并于切卵巢后不同时间取血,测定血清孕酮,以观察切卵巢后血清孕酮水平的变化。然后,各组分别于切除卵巢后不同时间开始补充外源性孕酮。于第九天处死,称胚珠、子宫和蜕膜的重量并做组织学检查,以观察切除卵巢后经过多长时间以及血清孕酮水平下降到什么程度将导致不可避免的流产,并分析通过干扰那些环节而导致流产。结果表明,切除卵巢后血清孕酮水平逐步下降,于切除卵巢后12小时,胚珠开始发生变化,此时血清孕酮水平为对照值的20%。切除卵巢后16小时,血清孕酮进一步下降,此时子宫及蜕膜重量才发生变化。这提示:“切除卵巢后12小时,当血清孕酮下降至对照水平的20%左右时,即可能引起胚珠的改变并导致流产”。为了观察切卵巢后蜕膜的变化,于大鼠假孕第七天切除卵巢,看到24小时后蜕膜重量降低并呈现组织学改变,但是在假孕第四天切除卵巢,12小时后即引起蜕膜改变。这说明,在假孕的不同时期,蜕膜对血清孕酮水平下降的敏感性不同。更多还原

【Abstract】 On Day 7 of pregnancy rats were ovariectomized and blood samples were collected to measure serum progesterone.After that,5 mg of progesteronewere given subcutaneusly daily.The animals were sacrificed on Day 9 ofpregnancy.At autopsy the conceptuses,uterus and decidua were separatelyweighed and sectioned and examined under microscope.It was found that the level of serum progesterone decreased progressivelyafter ovariectomy.At 12 hours after ovariectomy the conceptuses began tochange,at the same time,the level of serum progesterone was about 20% ofthe controls.However,the weight of the uterus and decidua only started todecrease at 16 hours after ovariectomy.Accordingly,it seemed that the changeof the conceptuses could be caused and abortion could be brought about whenthe level of serum progesterone decreased to about 20% of the controls.On Day 7 of pseudopregnancy rats were ovariectomized,after 24 hours,the weight of the decidua began to decrease.However,on Day 4 of pseudo-pregnancy,the weight of the dcidua did begin to decrease at 12 hours after ovariectomy.It was possible that on day 4 of pseudopregnancy the deciduawas more sensible to the decreasing of serum progerterone.更多还原