【摘要】 <正> 麦胚无细胞蛋白合成体系的特点是内源信息含量低,对外源RNA 反应灵敏,故在检测外源mRNA 和tRNA 的功能和蛋白质合成机制的研究中起着重要的作用。按Marcus 等的方法,制备无tRNA 的麦胚无细胞蛋白合成体系S_100-更多还原

【Abstract】 An exogenous RNA dependentcell-free protein synthesizing systemfrom wheat germ extract (S₂₆) hasbeen discribed.This system is pre-pared by simply passing through DEAE-cellulose （Cl-form） in the presence of0.3 M KCl and then by dialysingagainst a buffer containing 20%glycerol.The advantages of this cell-free system are as follows:1.The conventional method wassimplified by passing through DEAE-cellulose instead of ultracentrifuga-tion.2.The endogenous tRNA,mRNAand amino acids have been removedalmost completely,so that it becomesan exbgenous RNA dependent cell-free protein synthesizing system.3.The protein synthesizing acti-vity of the final preparation can bepreserved much longer in deep freezer（-20℃） than the system preparedbefore.更多还原