【摘要】 竹黄是腐生于我国南方剑竹上的一种子囊菌。子实体经分离后,菌丝接种,液体发酵。发酵液过滤,菌丝用沸水抽提,乙醇沉淀,去蛋白质,透析后即得粗制品。粗制品经DEAE-纤维素柱层析两次得到白色粉末的两个多醣组分,Sb₁及Sb₂。Sb₁及Sb₂分别经玻璃纤维纸高压电泳,凝胶柱层析,气相层析证明为一单一物质。Sb₁及Sb₂比旋度均为正值。Sb₁具890cm^-1的红外吸收。两者均不含氮。与碘无反应。经纸层析与气相色谱分析,Sb₁含D-葡萄糖,D-半乳糖和L-阿拉伯糖,其克分子比为0.37:1.0:0.07。Sb₂含D-葡萄糖,D-半乳糖,D-甘露糖和L-阿拉伯糖,其克分子比为0.25:1:0.47:0.12。更多还原

【Abstract】 Shiraia bambusicola （Henn.） is a saprophytic Ascomycetes on bamboo in south China. The fungus has been isolated from the fruiting body in pure form. The process of fermentation was carried out in tanks by conventional methods.Two polysaccharides, named Sb1 and Sb2, have been isolated from the mycelium. The crude polysaccharide fractions were obtained at first by chromatography on DEAE cellulose （CO₃⁼） column eluted with water. The crude product was taken up in a DEAE-cellulcse（B₄O₇⁼）column. Sb1 fraction was obtained by eluting. with H₂O, and Sb2 with 0.05 M Na₂B₄O₇ solution. Both fractions gave a single spot on glass-filter paper electrophoresis in alkaline borate buffer and gave a single peak on gel chromatography with Sephadex G-200. The moleoular weight of Sb1 and Sb2 were 6000 and 25000 respectively.Both Sb1 and Sb2 showed positive optioal rotations: Sb1: [a]_D^26.5+52.4° （C=1.1, H₂O）, Sb2: [a]_D³⁵+18.1°（C=1.2, H₂O）.The IR of Sb1 had an absorption band at 890 cm^-1 being due to β-glycosidic linkage.L-Ara, D-Glc and D-Gal were identified as component sugars of Sb1, and L-Ara, D-Man, D-Glc and D-Gal as components of Sb2.Quantitative analysis of Sb1 and Sb2 showed that the molar ratio of Glc:Gal:Ara is 0.37:1.0:0.07 and Glc: Gal:Man: Ara is 0.25:1.0:0.47:0.12 respectively.更多还原