【摘要】 高产青霉素酰化酶的大肠杆菌细胞用明胶包(?),并由戊二醛将其固定。这种固定化细胞用来连续水解苄基青霉素,在37℃使用103天没有发现损失酶活性。它们已被用于6-氨基青霉烷酸的工业生产,效果令人满意。在七个半月内使用285次,水解速率没有明显下降。研究了固定化大肠杆菌细胞的酶性质,并与相应的天然细胞作了比较。发现固定化细胞的青霉素酰化酶比天然细胞的更稳定。更多还原

【Abstract】 E. coli cells having high penicillin acylase activity were entrapped in gelatin gel and crosslinked with glutaraldehyde.The immobilized cells were used for the continuous hydrolysis of benzylpenicillin, no loss of enzymatic activity was found after 103 days at 37℃. They have been satisfactorily applied to industrial production of 6-aminopenicillanic acid, no significant reduction of the hydrolytic rate was observed after 285 cycles within seven and a half months.The enzymatic properties of immobilized E. coli cells have been investigated and compared with those of intact cells. It was found that penicillin acylase activity of the immobilized cells was more stable than that of the intact cells.更多还原